LLP789_αGCN4-DNMT3A-CO
(Plasmid
#211783)
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PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection
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Depositing Lab
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Depositing OrganizationUniversity of Western Australia
Located in Australia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211783 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 211783-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 211783-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
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Vector typeMammalian Expression
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameaGCN4-DNMT3A
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SpeciesH. sapiens (human)
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MutationLast 300 bp codon optimised (CO) to detect DNMT3A expression destinct from endogenous mRNA
- Promoter pEF1a and pSV40
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Tag
/ Fusion Protein
- 3xTy1 (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer -
- 3′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.
DNA (Catalog # 211783-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 211783-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP789_αGCN4-DNMT3A-CO was a gift from Ryan Lister (Addgene plasmid # 211783 ; http://n2t.net/addgene:211783 ; RRID:Addgene_211783) -
For your References section:
A modular dCas9-based recruitment platform for combinatorial epigenome editing. Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R. Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. 10.1093/nar/gkad1108 PubMed 38000387
