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Identification of conserved RNA regulatory switches in living cells using RNA secondary structure ensemble mapping and covariation analysis.

Borovska I, Zhang C, Dulk SJ, Morandi E, Cardoso MFS, Bourkia BM, van den Homberg DAL, Wolfinger MT, Velema WA, Incarnato D
Nat Biotechnol. 2025 Jul 25. doi: 10.1038/s41587-025-02739-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID Plasmid Purpose
212594pET22b(+)-Ptac-Ecoli_lpxPIPTG-inducible construct for the expression of lpxP under control of the 5'UTR RNA thermometer

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