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Modular Peroxidase-Based Reporters for Detecting Protease Activity and Protein Interactions with Temporal Gating.
Zhou G, Wan WW, Wang W
J Am Chem Soc. 2022 Dec 21;144(50):22933-22940. doi: 10.1021/jacs.2c08280. Epub 2022 Dec 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
213405CLAPonPeroxidase (APEX) reporter for TEV protease cleavage
213406β2AR-PPI-CALPon1.0 (APEX)Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)
213407β2AR-PPI-CALPon1.0 (TEVp)Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)
213408Ca-LiPPI-CLAPon1.0 (APEX)Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)
213409Ca-LiPPI-CLAPon1.0 (TEVp)Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

Antibodies from Article