Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists
Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
bioRxiv 2024.04.19.590338 Article
Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
bioRxiv 2024.04.19.590338 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214983 | pLV UBC APEX2-3xFLAG-IRES-eGFP | Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments). |
| 214990 | mNeonGreen-P2A-3xFLAG-msEEA1 HDR template | Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting. |
| 214991 | pTwist-msEEA1 | Gateway entry vector with mouse EEA1 |
| 214994 | pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1 | Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels. |
| 214995 | pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1 | Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels. |
| 214996 | pLV UBC hTMEM115-3C-3xHA-P2A-eGFP | Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels. |
| 214997 | pLV UBC msTMEM115-3C-3xHA-P2A-eGFP | Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels. |
| 214998 | hTMEM115-TEV-3xHA-P2A-mScarlet HDR template | Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting. |
| 214999 | hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template | Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting. |
| 215000 | pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template | Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting. |
| 218633 | pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation of msNLRP3 |
| 218634 | pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation of msNLRP3(ΔPYD, I125M-END) |
| 218635 | pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E) | Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E) |
| 218636 | pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A) | Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A) |
| 218637 | pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation control of OSBP(PH domain) |
| 218638 | pDONR221 msNLRP3 (STOP) | Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional) |
| 218640 | pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP) | Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon) |
| 218641 | pDONR221 msNLRP3(ΔPYD, I125M-END) | Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon) |
| 218642 | pLenti UBC hNLRP3-mNG | Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection |
| 218643 | pHAGE pGK hNLRP3-mNG | Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection |
| 218644 | pDONR221 hNLRP3-mNG-STOP | Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon) |
| 218645 | pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen | Gateway shuttling vector for APEX2-tagged P4C, a P4C biosensor |
| 218647 | pDONR221 hNLRP3 R262W (disease-associated mutation) | Gateway shuttling vector for cloning NLRP3-R262W (no stop codon) |
| 218648 | msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti | Knockout vector for mouse Plekha3 (Fapp1) |
| 218649 | msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti | Knockout vector for mouse Plekha3 (Fapp1) |
| 218650 | msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti | Knockout vector for mouse Plekha8 (Fapp2) |
| 218651 | msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti | Knockout vector for mouse Plekha8 (Fapp2) |
| 218653 | msHook2 g2 lentiCRISPRv2-mCherry | Knockout vector for mouse Hook2 |
| 218654 | msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry | Knockout vector for mouse Fam160b1 (Fhip2A) |
| 218655 | msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry | Knockout vector for mouse Fam160b1 (Fhip2A) |
| 218656 | PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti | Knockout vector for human PLEKHA3 (FAPP1) |
| 218657 | PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti | Knockout vector for human PLEKHA3 (FAPP1) |
| 218658 | PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti | Knockout vector for human PLEKHA8 (FAPP2) |
| 218659 | PLEKHA8 (FAPP2) g2 lentiCRISPRv2-opti | Knockout vector for human PLEKHA8 (FAPP2) |
| 218660 | TMEM165 g1 LentiCRISPRv2-mCherry | Knockout vector for human TMEM165 |
| 218661 | TMEM165 g2 LentiCRISPRv2-mCherry | Knockout vector for human TMEM165 |
| 218662 | GOLIM4 g1 LentiCRISPRv2-mCherry | Knockout vector for human GOLIM4 (GPP130/GOLPH4) |
| 218663 | GOLIM4 g2 LentiCRISPRv2-mCherry | Knockout vector for human GOLIM4 (GPP130/GOLPH4) |