A sensitive orange fluorescent calcium ion indicator for imaging neural activity.
Aggarwal A, Baker HA, Durst CD, Chen IW, de Chambrier P, Stine C, Gonzales JM, Marvin JS, Vandal M, Lundberg T, Brown-Panton CA, Sakoi K, Patel R, Wang CY, Visser F, Fouad Y, Sunil S, Wiens M, Terai T, Takahashi-Yamashiro K, Thompson RJ, Brown TA, Nasu Y, Nguyen MD, Gordon GRJ, McFarlane S, Podgorski K, Holtmaat A, Campbell RE, Lohman AW
Nat Commun. 2026 Jun 22. doi: 10.1038/s41467-026-74553-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224932 | pRSET OCaMP | Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope) |
| 224934 | pAAV-hSYN1-NES-OCaMP-WPRE | pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide |
| 224936 | pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE | pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter |
| 229853 | pAAV-hSyn-FLEX(cre)-OCaMP-WPRE | pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter |