Structure-guided engineering of a fast genetically encoded sensor for real-time H(2)O(2) monitoring.
Lee JD, Won W, Kimball K, Wang Y, Yeboah F, Evitts KM, Neiswanger C, Schattauer S, Rappleye M, Bremner SB, Chun C, Smith N, Mack DL, Young JE, Lee CJ, Chavkin C, Berndt A
bioRxiv [Preprint]. 2024 Feb 4:2024.01.31.578117. doi: 10.1101/2024.01.31.578117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216111 | pC3.1_CMV_oROS-G | Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells. |
| 216112 | pC3.1_CMV_oROS-G_LF(C199S) | Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells. |
| 216113 | pC3.1_CAG_oROS-Gr | Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells. |
| 216114 | pC3.1_CAG_oROS-Gr_LF(C199S) | Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells. |
| 216115 | AAV2_CAG_oROS-G | Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors. |
| 216116 | AAV2_CAG_oROS-G_LF(C199S) | Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors. |