A toolkit for testing membrane-localising tags across species
Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken J, Leclere L, Averof M
bioRxiv 2024.11.12.623055
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225927 | 0_T7-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control) |
| 225928 | 1_T7-mScarlet3-Hras | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal |
| 225929 | 2_T7-mScarlet3-KRas | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal |
| 225930 | 3_T7-mScarlet3-KRas6R | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal |
| 225931 | 4_T7-mScarlet3-RitC | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal |
| 225932 | 5_T7-GAP43-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal |
| 225933 | 6_T7-Lyn-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal |
| 225934 | 7_T7-Src64B-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal |
| 225935 | 8_T7-PH-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal |
| 225936 | 9_T7-mScarlet3-LactC2 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal |
| 225937 | 10_T7-SP-CD8tm-mScarlet3 | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain |
| 229806 | 11_T7-SP-mScarlet3-GPI | In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site |