Adrian Liston Lab: Systematic in vivo testing identifies context- and tissue-dependent migratory requirements for regulatory and conventional T cells

Unpublished

These materials are included in the sgRNA FlowCode Plasmids Kit.

Plasmids from Article

ID	Plasmid	Purpose
231423	Flowcode_01	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs
231424	Flowcode_02	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231425	Flowcode_03	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231426	Flowcode_04	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231427	Flowcode_05	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231430	Flowcode_06	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231431	Flowcode_07	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231432	Flowcode_08	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231433	Flowcode_09	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231434	Flowcode_10	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231435	Flowcode_11	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231436	Flowcode_12	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231437	Flowcode_13	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231438	Flowcode_14	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231439	Flowcode_15	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231440	Flowcode_16	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231441	Flowcode_17	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231442	Flowcode_18	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231443	Flowcode_19	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231444	Flowcode_20	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231445	Flowcode_21	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231446	Flowcode_22	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231447	Flowcode_23	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231448	Flowcode_24	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231449	Flowcode_25	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231450	Flowcode_26	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231451	Flowcode_27	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231452	Flowcode_28	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231453	Flowcode_29	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231454	Flowcode_30	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231455	Flowcode_31	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231456	Flowcode_32	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231457	Flowcode_33	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231458	Flowcode_34	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231459	Flowcode_35	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231460	Flowcode_36	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231461	Flowcode_37	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231468	Flowcode_38	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231469	Flowcode_39	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231470	Flowcode_40	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231471	Flowcode_41	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231472	Flowcode_42	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231473	Flowcode_43	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231474	Flowcode_44	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231475	Flowcode_45	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231476	Flowcode_46	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231477	Flowcode_47	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231478	Flowcode_48	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231479	Flowcode_49	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231480	Flowcode_50	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231481	Flowcode_51	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231482	Flowcode_52	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231483	Flowcode_53	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231484	Flowcode_54	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231485	Flowcode_55	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
231486	Flowcode_56	Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.