Flowcode_11
(Plasmid #231435)

• Purpose: (Empty Backbone) Empty backbone which expresses a unique 3 epitope tag on the histone H2bc3. This can be used for CRISPR knockdown of genes via ligation of gRNA oligos downstream of the U6 promoter. This plasmid is to be used with gRNAs.
• Depositing Lab: Adrian Liston
• Publication: Systematic in vivo testing identifies context- and tissue-dependent migratory requirements for regulatory and conventional T cells (unpublished)

Backbone

• Vector backbone: pMSCV U6 iScaffold H2bc3
• Backbone manufacturer: Liston-Dooley Lab
• Backbone size (bp): 6658
• Vector type: Mammalian Expression, Retroviral
• Promoter: hU6
• Tags / Fusion Proteins:
  • AU1
  • FLAG
  • V5

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmids were generated using pro-code vectors received from Dr Brian Brown at Mount Sinai and we have their permission via email for these to be deposited.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please refer to the Wroblewska et al. paper "Protein barcodes enable high-dimensional single cell CRISPR screens" and the Brown lab Pro-Code vector kit page (#1000000177, #1000000197) at https://www.addgene.org/kits/brown-pro-code/ for further protocols.