Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius.
Paredes-Barrada M, Mathissen A, van der Molen RA, Jimenez-Huesa PJ, Polano ME, Donati S, Abele M, Ludwig C, van Kranenburg R, Claassens NJ
Metab Eng. 2025 Sep;91:145-157. doi: 10.1016/j.ymben.2025.04.002. Epub 2025 Apr 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231850 | pJS15A-TCas9-adhPKO-sp3 | KO of adhP in Parageobacillus thermoglucosidasius |
| 231852 | pJS15A-pRpsl8-adhT | Expression of adhT (from Parageobacillus thermoglucosidasius) under the control of Rpsl8 promoter |
| 231853 | pJS15A-pLDH-adhT | Expression of adhT (from Parageobacillus thermoglucosidasius) under the control of pLDH promoter |
| 231854 | pZASS-adhT | Expression of adhT (from Parageobacillus thermoglucosidasius) |