Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases.
Qianzhu H, Tan YJ, Abdelkader EH, Huber T, Otting G
ACS Sens. 2025 Apr 25;10(4):3152-3161. doi: 10.1021/acssensors.5c00432. Epub 2025 Apr 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232253 | pRSF-G1(2FF)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of 2-Fluoro-L-Phenylalanine (2FF) into proteins in E. coli in response to the amber (TAG) codon |
| 232254 | pRSF-G1(4FF)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Phenylalanine (4FF) into proteins in E. coli in response to the amber (TAG) codon |
| 232255 | pRSF-G1(F5F)RS | tRNA synthetase/tRNA pair for the in vivo incorporation of pentaFluoro-L-Phenylalanine (F5F) into proteins in E. coli in response to the amber (TAG) codon |