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Red-shifted GRAB acetylcholine sensors for multiplex imaging in vivo.

Xie S, Miao X, Li G, Zheng Y, Li M, Ji E, Wang J, Li S, Cai R, Geng L, Feng J, Wei C, Li Y
Nat Neurosci. 2026 Jun 16. doi: 10.1038/s41593-026-02325-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234412pAAV-hSyn-GRAB-rACh1hExpresses the red ACh sensor GRAB rACh1h in neurons
234413pAAV-hSyn-GRAB-rACh1mExpresses the red ACh sensor GRAB rACh1m in neurons
234414pAAV-hSyn-GRAB-rACh1lExpresses the red ACh sensor GRAB rACh1l in neurons
234415pAAV-hSyn-GRAB-rAChmutExpresses the red ACh sensor GRAB rAChmut in neurons
234416pDisplay-GRAB-rACh1h-IRES-EGFP-CAAXExpresses the red ACh sensor GRAB rACh1h and a membrane-localized EGFP in mammalian cells
234418pDisplay-GRAB-rACh1l-IRES-EGFP-CAAXExpresses the red ACh sensor GRAB rACh1l and a membrane-localized EGFP in mammalian cells
234419pDisplay-GRAB-rAChmut-IRES-EGFP-CAAXExpresses the red ACh sensor GRAB rAChmut and a membrane-localized EGFP in mammalian cells
251360pAAV-ef1a-DIO-rACh1hExpresses the cre dependent red ACh sensor GRAB rACh1h in neurons

Antibodies from Article