CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation.
Cajka G, Liu M, Shalem O
bioRxiv [Preprint]. 2024 Oct 19:2024.10.19.619045. doi: 10.1101/2024.10.19.619045.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254798 | pLV_CROPseq_v2-2xMycNLS-mScarlet | Plasmid backbone for guide cloning |
| 254799 | pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |
| 254800 | pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-ZIM3-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of dCas9-ZIM3 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |
| 254801 | pLV_hPGK-2xMeCP2NLS-Cas9-HA-2xSV40NLS-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of spCas9 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |