CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation.
Cajka G, Liu M, Shalem O
bioRxiv [Preprint]. 2024 Oct 19:2024.10.19.619045. doi: 10.1101/2024.10.19.619045.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254790 | pPB_UCOE-CAG-dCas9-HA-2xSV40NLS-TagBFP-KOX1-NpNLS-rBGpA_chs4_EF1a-Blast-rBGpA | Constituitve expression of dCas9-KOX1 via transposase integration |
| 254791 | pPB_UCOE-CAG-Cas9-NpNLS-FLAG-TagBFP-2xSV40NLS-rBGpA_chs4_EF1a-Blast-rBGpA | Constituitve expression of spCas9 via transposase integration |
| 254792 | pPB_UCOE-CAG-2xMeCP2NLS-dCas9-HA-2xSV40NLS-TagBFP-KOX1_NpNLS-rBGpA_chs4_EF1a-Blast-rBGpA | Constitutive expression of dCas9-KOX1 with optimized nuclear localization for iPSC-derived neurons via transposase integration |
| 254793 | pPB_UCOE-CAG-2xMeCP2NLS-Cas9-NpNLS-FLAG-TagBFP-2xSV40NLS-rBGpA_chs4_EF1a-Blast-rBGpA | Constituitve expression of spCas9 with optimized nuclear localization for iPSC-derived neurons via transposase integration |
| 254794 | pPB_UCOE-CAG-noNLS-dCas9-HA-TagBFP-KOX1-rBGpA_chs4_EF1a-Blast-rBGpA | Negative control for nuclear localization of dCas9-KOX1 expressed via transposase integration |
| 254795 | pPB_UCOE-CAG-NpNLS-2xSV40NLS-dCas9-HA-TagBFP-KOX1-rBGpA_chs4_EF1a-Blast-rBGpA | Constituitve expression of dCas9-KOX1 via transposase integration |
| 254796 | pPB_UCOE-CAG-2xMycNLS-2xSV40NLS-dCas9-HA-TagBFP-KOX1-rBGpA_chs4_EF1a-Blast-rBGpA | Constituitve expression of dCas9-KOX1 via transposase integration |
| 254797 | pPB_UCOE-CAG-2xMeCP2NLS-dCas9-HA-TagBFP-KOX1-rBGpA_chs4_EF1a-Blast-rBGpA | Minimal construct for constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via transposase integration |
| 254798 | pLV_CROPseq_v2-2xMycNLS-mScarlet | Plasmid backbone for guide cloning |
| 254799 | pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |
| 254800 | pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-ZIM3-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of dCas9-ZIM3 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |
| 254801 | pLV_hPGK-2xMeCP2NLS-Cas9-HA-2xSV40NLS-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE | Constituitve expression of spCas9 with optimized nuclear localization in iPSC-derived neurons via lenti delivery |