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pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-ZIM3-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE
(Plasmid #254800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254800 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Mammalian Gene Expression Lentiviral Vector
  • Backbone manufacturer
    VectorBuilder
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    mTagBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xMeCP2NLS-dCas9-HA-2xSV40NLS-ZIM3-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS
  • Species
    Synthetic
  • Mutation
    catalytically dead spCas9
  • Promoter hPGK

Cloning Information

  • Cloning method Other

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.19.619045 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-ZIM3-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE was a gift from Ophir Shalem (Addgene plasmid # 254800 ; http://n2t.net/addgene:254800 ; RRID:Addgene_254800)

  • For your References section:

    CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation. Cajka G, Liu M, Shalem O. bioRxiv [Preprint]. 2024 Oct 19:2024.10.19.619045. doi: 10.1101/2024.10.19.619045. 10.1101/2024.10.19.619045 PubMed 39464063
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