A Modular Optogenetic Split-Small GTPase Platform Reveals Long-Range RhoA Signaling.
Faulkner B, He Y, Sitrin D, Ziamanesh L, Stains CI
ACS Chem Biol. 2026 Oct 23. doi: 10.1021/acschembio.6c00691.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 243661 | pIRES-tagRFPt-SspB Nano-Cdc42/N12-Cdc42/13C(Q61L)-iLID-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Nano-iLID components and FPs |
| 243662 | pIRES-tagRFPt-SspB Micro-Cdc42/N12-Cdc42/13C(Q61L)-iLID-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Micro-iLID components and FPs |
| 243663 | pIRES-tagRFPt-SspB Milli-Cdc42/N12-Cdc42/13C(Q61L)-iLID-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Milli-iLID components and FPs |
| 243664 | pIRES-tagRFPt-SspB Nano-Cdc42/N12-Cdc42/13C(Q61L)-iLID C252A-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Nano-iLID C252A components and FPs |
| 243665 | pIRES-tagRFPt-SspB Micro-Cdc42/N12-Cdc42/13C(Q61L)-iLID C252A-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Micro-iLID C252A components and FPs |
| 243666 | pIRES-tagRFPt-SspB Milli-Cdc42/N12-Cdc42/13C(Q61L)-iLID C252A-mVenus-CAAX | Express split-Cdc42 fragments (Cdc42/N12-Cdc42/13C(Q61L)) fused with SspB Milli-iLID C252A components and FPs |
| 243667 | pIRES-tagRFPt-SspB Micro-Rac1/N12-Rac1/13C(Q61L)-iLID-mVenus-CAAX | Express split-Rac1 fragments (Rac1/N12-Rac1/13C(Q61L)) fused with SspB Micro-iLID components and FPs |
| 243668 | pIRES-tagRFPt-SspB Micro-Rac1/N12-Rac1/13C(Q61L)-iLID C252A-mVenus-CAAX | Express split-Rac1 fragments (Rac1/N12-Rac1/13C(Q61L)) fused with SspB Micro-iLID C252A components and FPs |
| 243669 | pIRES-tagRFPt-SspB Micro-RhoA/N14-RhoA/15C(Q63L)-iLID-mVenus-CAAX | Express split-RhoA fragments (RhoA/N14-RhoA/15C(Q63L)) fused with SspB Micro-iLID components and FPs |
| 243670 | pIRES-tagRFPt-SspB Micro-RhoA/N14-RhoA/15C(Q63L)-iLID C252A-mVenus-CAAX | Express split-RhoA fragments (RhoA/N14-RhoA/15C(Q63L)) fused with SspB Micro-iLID C252A components and FPs |