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pIRES-tagRFPt-SspB Milli-Cdc42/N12-Cdc42/13C(Q61L)-iLID-mVenus-CAAX
(Plasmid #243663)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243663 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 6105
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tagRFPt-SspB Milli-Cdc42/N12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1230
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter CMV
  • Tag / Fusion Protein
    • tagRFPt (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Mlu1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cdc42/13C(Q61L)-iLID-mVenus-CAAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1830
  • Mutation
    Cdc42-Q61L
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer IRES-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid will express two separate inserts, which include split-GTPase fragments that are fused with iLID components and FPs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-tagRFPt-SspB Milli-Cdc42/N12-Cdc42/13C(Q61L)-iLID-mVenus-CAAX was a gift from Cliff Stains (Addgene plasmid # 243663 ; http://n2t.net/addgene:243663 ; RRID:Addgene_243663)

  • For your References section:

    A Modular Optogenetic Split-Small GTPase Platform Reveals Long-Range RhoA Signaling. Faulkner B, He Y, Sitrin D, Ziamanesh L, Stains CI. ACS Chem Biol. 2026 Oct 23. doi: 10.1021/acschembio.6c00691. 10.1021/acschembio.6c00691 PubMed 42922161
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