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Balancing off-target and on-target considerations for optimized CRISPR-Cas9 knockout library design.

Drepanos LM, Srikanth S, Kaplan EG, Shah ST, Velasco BE, Merzouk S, Doench JG
Cell Genom. 2026 May 13;6(5):101190. doi: 10.1016/j.xgen.2026.101190. Epub 2026 Mar 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247026Jacquere (split-vector)Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.
247027Jacquere (all-in-one vector)Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.
247028Julianna (split-vector)Enriched for effective guides through prioritization of guides with strong on-target activity. Designed against current gene annotations, offering coverage of the contemporary mouse genome.

Antibodies from Article