A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes.
Slobodin B, Gerst JE
RNA. 2010 Nov;16(11):2277-90. doi: 10.1261/rna.2091710. Epub 2010 Sep 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227819 | pNX24-RFP-MS2SL | Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene |
| 249048 | pcDNA4-MS2-CP-GFP-SBP | Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP) |