pNX24-RFP-MS2SL
(Plasmid
#227819)
-
PurposeExpresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
-
Depositing Lab
-
Depositing OrganizationWeizmann Institute of Science
Located in Israel -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 227819-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 227819-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3.1
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)Stbl3
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert name3xHA-RFP-24xMS2SL
-
SpeciesSynthetic
-
Insert Size (bp)2128
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer -
- (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameNeo resistance
-
SpeciesSynthetic
-
Insert Size (bp)792
- Promoter SV40
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.
Please note: This plasmid is prone to recombination and removal of some of the MS2 repeats. Please select multiple colonies for screening for the full length plasmid before performing experiments.
DNA (Catalog # 227819-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 227819-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pNX24-RFP-MS2SL was a gift from Jeffrey Gerst (Addgene plasmid # 227819 ; http://n2t.net/addgene:227819 ; RRID:Addgene_227819) -
For your References section:
A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes. Slobodin B, Gerst JE. RNA. 2010 Nov;16(11):2277-90. doi: 10.1261/rna.2091710. Epub 2010 Sep 28. 10.1261/rna.2091710 PubMed 20876833
