Establishing synthetic ribbon-type active zones in a heterologous expression system.
Kapoor R, Do TT, Schwenzer N, Petrovic A, Dresbach T, Lehnart SE, Fernandez-Busnadiego R, Moser T
Elife. 2026 Jan 13;13:RP98254. doi: 10.7554/eLife.98254.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241988 | pAAV-CMV-HBA-RIBEYE-eGFP | Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance |
| 241989 | f(syn)w-CMV-mKate2-p2A-RimBP2 | Insert coding for mouse RBP2 (accession ID NP_001074857.1) with an N-terminal mKATE2 with a p2A cleavage site cloned inframe into a f(syn)w-mKATE2-p2A. |
| 241990 | pAAV-CMV-RBP2 | Mouse RBP2 (accession ID NP_001074857.1), CMV promoter-enhancer, downstream WPRE. |
| 250160 | Full Length Rat Bassoon | Expresses Rat Bassoon in mammalian cells |
| 250161 | palm-Bassoon-GFP | Encodes a version of rat Bassoon with a C-terminal GFP-tag and with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression. |
| 250162 | palm-Bassoon | Encodes a version of rat Bassoon with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression. |