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Directed evolution of a genetically encoded photocatalyst for temporally resolved proximity labeling of subcellular RNAs and proteins

Fang Y, Ren Z, Zheng F, Wang R, Zhao S, Wang W, Li C, Liu-Yang L, Lin C, Zou P
Research Square 2025 [Preprint]. https://doi.org/10.21203/rs.3.rs-6890342/v1 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
250916pLX304-Lantern-Sec61βExpresses Lantern fused to Sec61β in mammalian cells for targeting to the ER
250917pLX304-Lantern-MAVSExpresses Lantern fused to the C-terminal 31 amino acids of MAVS in mammalian cells for targeting to the outer mitochondrial membrane
250918pLX304-G3BP1-LanternExpresses Lantern fused to G3BP1 in mammalian cells for targeting to stress granules
250919pLX304-Lantern-HaloTag-NESExpresses Lantern in mammalian cytosol
250920pLX304-mito-LanternExpresses Lantern fused to the N-terminal 23 amino acid residues of human COX4 in mammalian cells
250922pcDNA3.1-mito-LanternExpresses Lantern fused to the N-terminal 23 amino acid residues of human COX4 in mammalian cells

Antibodies from Article