Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.
Keates T, Cooper CD, Savitsky P, Allerston CK, Phillips C, Hammarstrom M, Daga N, Berridge G, Mahajan P, Burgess-Brown NA, Muller S, Graslund S, Gileadi O
N Biotechnol. 2012 Jun 15;29(5):515-25. doi: 10.1016/j.nbt.2011.10.007. Epub 2011 Oct 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250993 | pCDF-BirA | Vector for co-expression of BirA for in-vivo biotinylation of Avi-tagged proteins. Spectinomycin-resistant, compatible with pET and pACYC vectors. Expression of BirA is driven by T7/Lac promoter. |