pCDF-BirA
(Plasmid
#250993)
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PurposeVector for co-expression of BirA for in-vivo biotinylation of Avi-tagged proteins. Spectinomycin-resistant, compatible with pET and pACYC vectors. Expression of BirA is driven by T7/Lac promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDF-Duet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF-BirA was a gift from Opher Gileadi (Addgene plasmid # 250993 ; http://n2t.net/addgene:250993 ; RRID:Addgene_250993) -
For your References section:
Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation. Keates T, Cooper CD, Savitsky P, Allerston CK, Phillips C, Hammarstrom M, Daga N, Berridge G, Mahajan P, Burgess-Brown NA, Muller S, Graslund S, Gileadi O. N Biotechnol. 2012 Jun 15;29(5):515-25. doi: 10.1016/j.nbt.2011.10.007. Epub 2011 Oct 19. 10.1016/j.nbt.2011.10.007 PubMed 22027370