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(Plasmid #133283)


Item Catalog # Description Quantity Price (USD)
Plasmid 133283 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8901
  • Total vector size (bp) 9618
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    Aequorea victoria
  • Insert Size (bp)
  • GenBank ID
  • Promoter MoMuLV
  • Tag / Fusion Protein
    • Humanized wild type biotin ligase BirA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTCTGGCTAACTAGAGAAC
  • 3′ sequencing primer AGACGGCAATATGGTGGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Please note that the plasmid map and some versions of SnapGene incorrectly identify BirA as a mutant. We have verified that this sequence is the WT BirA gene. Addgene is working to fix this issue.

1. Kulyyassov, A. et al. PUB-MS: A Mass Spectrometry-based Method to Monitor Protein-Protein Proximity in vivo. Journal of Proteome Research10, 4416-4427, doi:10.1021/pr200189p (2011).
2. Shoaib, M. et al. PUB-NChIP-"in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Research23, 331-340, doi:10.1101/gr.134874.111 (2013).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOz-humBirA-GFP was a gift from Arman Kulyyassov (Addgene plasmid # 133283 ; ; RRID:Addgene_133283)
  • For your References section:

    In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation. Kulyyassov A, Ogryzko V. Biomolecules. 2020 Jan 16;10(1). pii: biom10010142. doi: 10.3390/biom10010142. 10.3390/biom10010142 PubMed 31963153