The Escherichia coli replication initiator DnaA is titrated on the chromosome.
Olivi L, Kostlbacher S, Ludwig C, Langendoen M, Claassens NJ, Ettema TJG, van der Oost J, Ten Wolde PR, Hohlbein J, Staals RHJ
Nat Commun. 2025 Aug 21;16(1):7813. doi: 10.1038/s41467-025-63147-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212605 | pTarget_dnaA-PAmCherry2.1 | Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein PAmCherry2(M10L), together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene. |
| 212607 | pTarget_dnaA-mEos4b | Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein mEos4b, together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene. |