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U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations.

Walter DM, Cho K, Sivakumar S, Denney D, Lee IT, Dohlman AB, Heinz JM, Shurberg E, Jiang KX, Gupta AA, Frampton GM, Meyerson M
bioRxiv [Preprint]. 2025 Sep 18:2025.03.21.644128. doi: 10.1101/2025.03.21.644128. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
256552pLX301-U2AF1-WT-FLAGLentiviral vector for expression of FLAG-tagged U2AF1 in mammalian cells
256553pLX301-U2AF1-S34F-FLAGLentiviral vector for expression of FLAG-tagged U2AF1 S34F in mammalian cells
256554pLX301-U2AF1-I24T-FLAGLentiviral vector for expression of FLAG-tagged U2AF1 I24T in mammalian cells
256555pSpliceExpress-KRAS-Exon2pSpliceExpress splicing reporter to measure inclusion or skipping of exon 2 of KRAS
256556pSpliceExpress-KRAS-Exon3pSpliceExpress splicing reporter to measure inclusion or skipping of exon 3 of KRAS

Antibodies from Article