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pLX301-U2AF1-WT-FLAG
(Plasmid #256552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256552 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLX301
  • Total vector size (bp) 8595
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U2AF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    744
  • Entrez Gene
    U2AF1 (a.k.a. FP793, RN, RNU2AF1, U2AF35, U2AFBP)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Root (Addgene #25895)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX301-U2AF1-WT-FLAG was a gift from Matthew Meyerson (Addgene plasmid # 256552 ; http://n2t.net/addgene:256552 ; RRID:Addgene_256552)

  • For your References section:

    U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations. Walter DM, Cho K, Sivakumar S, Denney D, Lee IT, Dohlman AB, Heinz JM, Shurberg E, Jiang KX, Gupta AA, Frampton GM, Meyerson M. bioRxiv [Preprint]. 2025 Sep 18:2025.03.21.644128. doi: 10.1101/2025.03.21.644128. 10.1101/2025.03.21.644128 PubMed 40196662
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