Dominik Niopek Lab: Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites.
Wolf B, Shehu P, Brenker L, von Bachmann AL, Kroell AS, Southern N, Holderbach S, Eigenmann J, Aschenbrenner S, Mathony J, Niopek D
Nat Methods. 2025 Aug;22(8):1698-1706. doi: 10.1038/s41592-025-02741-z. Epub 2025 Aug 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231574 | PAC_E83_LOV_eGFP | This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light |
| 231575 | BLA_CAT_K136_GPG_LOV2 | This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light |
| 231576 | dSpyCas9-E566-GS-LOV2-VPR | Potent light-deactivated CRISPRa |
| 231577 | MbCas12a_K487_GPG_GR2 | Encodes a cortisol inducible variant of MbCas12a |
| 231578 | MbCas12a_N1153_GPG_GR2 | Encodes a cortisol inducible variant of MbCas12a |