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MbCas12a_N1153_GPG_GR2
(Plasmid #231578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231578 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 231578-D50 50 μg of DNA in Tris buffer $495
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DNA 231578-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    custom
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MbCas12a with a GR2 insertion behind N1153
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.06.565805 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MbCas12a_N1153_GPG_GR2 was a gift from Dominik Niopek (Addgene plasmid # 231578 ; http://n2t.net/addgene:231578 ; RRID:Addgene_231578)

  • For your References section:

    Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites. Wolf B, Shehu P, Brenker L, von Bachmann AL, Kroell AS, Southern N, Holderbach S, Eigenmann J, Aschenbrenner S, Mathony J, Niopek D. Nat Methods. 2025 Aug;22(8):1698-1706. doi: 10.1038/s41592-025-02741-z. Epub 2025 Aug 4. 10.1038/s41592-025-02741-z PubMed 40759748
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