LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size.
Serra-Vinardell J, Sandler MB, De Pace R, Manzella-Lapeira J, Cougnoux A, Keyvanfar K, Introne WJ, Brzostowski JA, Ward ME, Gahl WA, Sharma P, Malicdan MCV
Cell Mol Life Sci. 2023 Jan 28;80(2):53. doi: 10.1007/s00018-023-04695-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253243 | PGK-LAMP1-3xFLAG-APEX2 | Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection. |
| 253248 | PB-AlfaTag | Empty plasmid for cloning or as control for PB-LYST-AlfaTag (Plasmid #253252) and PB-AlfaTag-MYO5A (Plasmid #253250) |
| 253252 | PB-LYST-AlfaTag | Overexpress LYST with a C-terminal AlfaTag. |