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PB-LYST-AlfaTag
(Plasmid #253252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253252 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PB-3xFLAG-2xStrepTag (Addgene # 253239)
  • Backbone size w/o insert (bp) 12659
  • Vector type
    Mammalian Expression ; PiggyBac Transposon
  • Selectable markers
    Hygromycin ; mycNLS-mNeonGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LYST
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    11403
  • Entrez Gene
    LYST (a.k.a. CHS, CHS1, Mauve)
  • Promoter CAG
  • Tag / Fusion Protein
    • AlfaTag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-LYST-AlfaTag was a gift from May Malicdan (Addgene plasmid # 253252 ; http://n2t.net/addgene:253252 ; RRID:Addgene_253252)

  • For your References section:

    LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size. Serra-Vinardell J, Sandler MB, De Pace R, Manzella-Lapeira J, Cougnoux A, Keyvanfar K, Introne WJ, Brzostowski JA, Ward ME, Gahl WA, Sharma P, Malicdan MCV. Cell Mol Life Sci. 2023 Jan 28;80(2):53. doi: 10.1007/s00018-023-04695-x. 10.1007/s00018-023-04695-x PubMed 36707427
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