The dual ubiquitin binding mode of SPRTN secures rapid spatiotemporal proteolysis of DNA-protein crosslinks.
Song W, Zhao Y, Ruggiano A, Redfield C, Newman JA, Zhu X, Garcia-Flores M, Cruz-Migoni A, Roddan R, Perez-Rafols A, McHugh PJ, Elliott PR, Ramadan K
Nucleic Acids Res. 2025 Jul 8;53(13):gkaf638. doi: 10.1093/nar/gkaf638.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253392 | pNIC28-Ub | Expression in E. coli of ubiquitin. |
| 253393 | pET-47b-N-His-Ub2-I44A | Expression in E. coli of linear di-ubiquitin carrying the I44A mutation. |
| 253394 | pET-47b-N-His-Ub2-I120A | Expression in E. coli of linear di-ubiquitin carrying the I120A mutation. |
| 253395 | pET-47b-N-His-Ub2-I44AI120A | Expression in E. coli of linear di-ubiquitin carrying the I44AI120A double mutation. |
| 253397 | pET-24-hexaUb-I44AI120A | Expression in E. coli of linear hexa-ubiquitin carrying the I44AI120A mutation, with His tag at C-terminus. |
| 253398 | pGEX-4T1-SPRTN-MIU (1-25) | Expression in E. coli of SPRTN-MIU domain. |
| 253399 | pGEX-4T1-SPRTN-deltaC (Lys241AsnfsX8) | Expression in E. coli of SPRTN-deltaC patient variant (Lys241AsnfsX8). |
| 253400 | pNIC28-SPRTN-UBZ (452-489) | Expression in E. coli of SPRTN-UBZ domain. |
| 253401 | pNIC28-SPRTN-UBZ (452-489), C456AC459A | Expression in E. coli of SPRTN-UBZ domain carrying the C456AC459A double mutation. |
| 253402 | pNIC28-SPRTN core (26-240) | Expression in E. coli of SPRTN 26-240 truncation. |
| 253403 | pNIC28-SPRTN core (26-240)-E112Q | Expression in E. coli of SPRTN 26-240 truncation, carrying the E112Q mutation. |
| 253404 | pNIC28-SPRTN core (26-240)-L38A | Expression in E. coli of SPRTN 26-240 truncation, carrying the L38A mutation. |
| 253405 | pNIC28-SPRTN core (26-240)-L99A | Expression in E. coli of SPRTN 26-240 truncation, carrying the L99A mutation. |
| 253406 | pGEX-6P1-SPRTN-ZBD-BR (151-245) | Expression in E. coli of SPRTN-ZBD-BR truncation. |
| 253407 | pCold I-SPRTN-PIP-UBZ (320-489) | Expression in E. coli of SPRTN-PIP-UBZ truncation. |
| 253408 | pCold I-mH1-Ccys (S191C) | Expression in E. coli of mouse H1 carrying the S191C mutation. |
| 253409 | pCold I-mH1-Ncys (M1-C-T2) | Expression in E. coli of a mouse H1 variant where an additional Cys was inserted between M1 and T2. |
| 253410 | pCold I-Ub-mH1-Ncys (M1-C-T2) | Expression in E. coli of a mouse H1 variant fused with His-tagged monoUb at N-terminus. An additional Cys was inserted between M1 and T2 in H1. |
| 253411 | pCold I-Ub4-mH1-Ncys (M1-C-T2) | Expression in E. coli of a mouse H1 variant fused with His-tagged linear-tetraUb at N-terminus. An additional Cys was inserted between M1 and T2 in H1. |
| 253412 | pCold I-2xISG15-C78S-mH1-Ncys (M1-C-T2) | Expression in E. coli of a mouse H1 variant fused with His-tagged linear-diISG15 at N-terminus. An additional Cys was inserted between M1 and T2 in H1. |
| 253413 | pGEX6P1-UbcH5c-S22R | Expression in E. coli of UBCH5C carrying the S22R mutation. |