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pNIC28-Ub
(Plasmid #253392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253392 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pNIC28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Alt name
    UBA80
  • Alt name
    RPS27A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Entrez Gene
    RPS27A (a.k.a. CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80, eS31)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The His tag can be removed by TEV.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-Ub was a gift from Kristijan Ramadan (Addgene plasmid # 253392 ; http://n2t.net/addgene:253392 ; RRID:Addgene_253392)

  • For your References section:

    The dual ubiquitin binding mode of SPRTN secures rapid spatiotemporal proteolysis of DNA-protein crosslinks. Song W, Zhao Y, Ruggiano A, Redfield C, Newman JA, Zhu X, Garcia-Flores M, Cruz-Migoni A, Roddan R, Perez-Rafols A, McHugh PJ, Elliott PR, Ramadan K. Nucleic Acids Res. 2025 Jul 8;53(13):gkaf638. doi: 10.1093/nar/gkaf638. 10.1093/nar/gkaf638 PubMed 40685547
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