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(Plasmid #186805)


Item Catalog # Description Quantity Price (USD)
Plasmid 186805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5632
  • Total vector size (bp) 6136
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    UBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)
  • Tag / Fusion Protein
    • His6-Tev (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UbE2D3 UniProt ID: P61077

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-Tev-UbE2D3 was a gift from Lalit Deshmukh (Addgene plasmid # 186805 ; ; RRID:Addgene_186805)
  • For your References section:

    Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. Nelson SL, Li Y, Chen Y, Deshmukh L. J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. 10.1021/jacs.3c01943 PubMed 37010382