Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins.
Nelson SL, Li Y, Chen Y, Deshmukh L
J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. PubMed Article

Plasmids from Article

ID Plasmid Purpose
186802Alpha-Synuclein-HisCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.
186803Twin-Strep(R)-Tev-GSGS-UbiquitinCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.
186804His-Tev-UbE1Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.
186805His-Tev-UbE2D3Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.
186806His-Tev-Nedd4LCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.
186807His-Tev-WWP2Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.
186808ALIX-His(1-868, P801G)Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).
189819GB1-His-Tev-ALIX-V (348-702)Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

Antibodies from Article