Alpha-Synuclein-His
(Plasmid
#186802)
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PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-11a
- Backbone size w/o insert (bp) 5629
- Total vector size (bp) 6082
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAlpha-Synuclein-His
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SpeciesH. sapiens (human)
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
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Tag
/ Fusion Protein
- His6 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alpha-Synuclein UniProt ID: P37840
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Alpha-Synuclein-His was a gift from Lalit Deshmukh (Addgene plasmid # 186802 ; http://n2t.net/addgene:186802 ; RRID:Addgene_186802) -
For your References section:
Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. Nelson SL, Li Y, Chen Y, Deshmukh L. J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. 10.1021/jacs.3c01943 PubMed 37010382