RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity.

Dmytrenko O, Yuan B, Crosby KT, Krebel M, Chen X, Nowak JS, Chramiec-Glabik A, Filani B, Gribling-Burrer AS, van der Toorn W, von Kleist M, Achmedov T, Smyth RP, Glatt S, Bravo JPK, Heinz DW, Jackson RN, Beisel CL
Nature. 2026 Jan;649(8099):1312-1321. doi: 10.1038/s41586-025-09852-9. Epub 2026 Jan 7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
253627	CBS-6177	Expression of CAO1 target and CAO1 targeting spacer array
253628	CBS-6178	Expression of CAO1 target and GAPDH non-targeting spacer array
253629	CBS-6179	Expression of GAPDH target and GAPDH targeting spacer array
253630	CBS-6180	Expression of GAPDH target and CAO1 non-targeting spacer array
253631	CBS-6181	Expression of GFP target and GFP targeting spacer array
253632	CBS-6182	Expression of GFP target and CAO1 non-targeting spacer array
253633	CBS-7169	MpCas12a2 expression
253634	CBS-7170	MpCas12a expression
253635	CBS-7171	Ba1Cas12a3 expression
253636	CBS-6266	dBa1Cas12a3 expression
253637	CBS-7172	Sm3Cas12a3 expression
253638	CBS-7173	ca23Cas12a3 expression
253639	CBS-7174	ApCas12a4 expression