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CBS-7174
(Plasmid #253639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253639 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-29b(+)
  • Backbone manufacturer
    Twist
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ApCas12a4 E. coli codon optimized C-terminal His tag
  • Species
    Other
  • Mutation
    N/A
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBS-7174 was a gift from Chase Beisel (Addgene plasmid # 253639 ; http://n2t.net/addgene:253639 ; RRID:Addgene_253639)

  • For your References section:

    RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity. Dmytrenko O, Yuan B, Crosby KT, Krebel M, Chen X, Nowak JS, Chramiec-Glabik A, Filani B, Gribling-Burrer AS, van der Toorn W, von Kleist M, Achmedov T, Smyth RP, Glatt S, Bravo JPK, Heinz DW, Jackson RN, Beisel CL. Nature. 2026 Jan;649(8099):1312-1321. doi: 10.1038/s41586-025-09852-9. Epub 2026 Jan 7. 10.1038/s41586-025-09852-9 PubMed 41501459
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