Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choice.
Savani MR, Li B, Smith BC, Gu W, Xiao Y, Baquer G, Shipman T, Oken SS, Manoj N, Zacharias LG, Puliyappadamba VT, Stopka SA, Regan MS, Levitt MM, Edgar CK, Hicks WH, Anand S, Martin-Sandoval MS, Winston R, S Patricio J, Johnson X, Tippetts TS, Shi DD, Lemoff A, Richardson TE, Zinn PO, Solmonson A, Mathews TP, Agar NYR, DeBerardinis RJ, Abdullah KG, McBrayer SK
Nat Metab. 2026 Apr 29. doi: 10.1038/s42255-026-01520-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253766 | pLenti-EF1alpha-DEST-IRES-Neo | Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES. |
| 253767 | pLV-EF1alpha-DEST-IRES-Hygro | Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to hygromycin selection by an IRES. |
| 253768 | pLV-EF1alpha-DEST-IRES-Neo | Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES. |
| 253769 | pLV-EF1alpha-DEST-IRES-Puro | Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to puromycin selection by an IRES. |
| 253770 | pLV-EF1alpha-DEST-IRES-EGFP | Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to EGFP by an IRES. |
| 253771 | pLV-hPGK-DEST-IRES-Hygro | Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to hygromycin selection by an IRES. |
| 253772 | pLV-hPGK-DEST-IRES-Neo | Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to neomycin selection by an IRES. |
| 253773 | pLV-hPGK-DEST-IRES-Puro | Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to puromycin selection by an IRES. |
| 253775 | pLV-UbC-DEST-IRES-Hygro | Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to hygromycin selection by an IRES. |
| 253776 | pLV-UbC-DEST-IRES-Neo | Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to neomycin selection by an IRES. |
| 253777 | pLV-UbC-DEST-IRES-Puro | Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to puromycin selection by an IRES. |
| 253778 | pLV-UbC-DEST-IRES-EGFP | Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to EGFP by an IRES. |
| 253780 | pLV-TRE-DEST-UbC-tTSrtTA-IRES-Neo | Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to neomycin selection by an IRES. |
| 253781 | pLV-TRE-DEST-UbC-tTSrtTA-IRES-Puro | Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to puromycin selection by an IRES. |
| 253782 | pLV-TRE-DEST-UbC-tTSrtTA-IRES-EGFP | Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to EGFP by an IRES. |
| 253783 | pLenti-EF1alpha-EV-IRES-Neo | pLenti lentiviral expression vector encoding empty vector linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253784 | pLenti-EF1alpha-FLAG-HA-CAD_WT-HA-IRES-Neo | pLenti lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253785 | pLenti-EF1alpha-FLAG-HA-CAD_S1900A-HA-IRES-Neo | pLenti lentiviral expression vector encoding FLAG- and HA-tagged human CAD bearing S1900A mutation linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253786 | pLenti-EF1alpha-FLAG-HA-CAD_S1900D-HA-IRES-Neo | pLenti lentiviral expression vector encoding FLAG- and HA-tagged human CAD bearing S1900D mutation linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253787 | pLV-EF1alpha-EV-IRES-Neo | pLV lentiviral expression vector encoding empty vector linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253788 | pLV-EF1alpha-FLAG-HA-CAD_WT-HA-IRES-Neo | pLV lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253789 | pLV-EF1alpha-FLAG-HA-CAD_S1900A-HA-IRES-Neo | pLV lentiviral expression vector encoding FLAG- and HA-tagged human CAD bearing S1900A mutation linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253790 | pLV-EF1alpha-FLAG-HA-CAD_S1900D-HA-IRES-Neo | pLV lentiviral expression vector encoding FLAG- and HA-tagged human CAD bearing S1900D mutation linked to neomycin selection by an IRES under a human EF1alpha promoter. |
| 253791 | pLenti-PGK-EV-Hygro | pLenti lentiviral expression vector encoding empty vector under a human PGK promoter. |
| 253792 | pLenti-PGK-FLAG-HA-CAD_WT-HA-Hygro | pLenti lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD under a human PGK promoter. |