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pLenti-EF1alpha-FLAG-HA-CAD_S1900D-HA-IRES-Neo
(Plasmid #253786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253786 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti-EF1alpha-DEST-IRES-Neo
  • Backbone manufacturer
    This study
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CAD
  • Species
    H. sapiens (human)
  • Mutation
    S1900D
  • Entrez Gene
    CAD (a.k.a. CDG1Z, DEE50, EIEE50, GATD4)
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • FLAG-HA (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF1alpha-FLAG-HA-CAD_S1900D-HA-IRES-Neo was a gift from Samuel McBrayer (Addgene plasmid # 253786 ; http://n2t.net/addgene:253786 ; RRID:Addgene_253786)

  • For your References section:

    Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choice. Savani MR, Li B, Smith BC, Gu W, Xiao Y, Baquer G, Shipman T, Oken SS, Manoj N, Zacharias LG, Puliyappadamba VT, Stopka SA, Regan MS, Levitt MM, Edgar CK, Hicks WH, Anand S, Martin-Sandoval MS, Winston R, S Patricio J, Johnson X, Tippetts TS, Shi DD, Lemoff A, Richardson TE, Zinn PO, Solmonson A, Mathews TP, Agar NYR, DeBerardinis RJ, Abdullah KG, McBrayer SK. Nat Metab. 2026 Apr 29. doi: 10.1038/s42255-026-01520-0. 10.1038/s42255-026-01520-0 PubMed 42056505
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