Daniel Kiss Lab: Purification of Recombinant RNase R
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254212 | His6-V5-RNaseR-87-725-D280N | RNase R (87-725-D280N mutant) is an inactive mutant of RNase R that has no RNA exonuclease activity. It has both an N-terminal 6x HIS tag and a V5 tag that are ahead of a TEV protease cleavage site. |
| 254213 | His6-V5-RNaseR-87-725 | RNase R (87-725 WT) is a highly active version of RNAse R. It specifically degrades linear RNAs with free 3' end. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site. |
| 254214 | His6-V5-RNaseR | His6-V5-RNaseR encodes full-size RNAse R. It specifically degrades linear RNAs with free 3' end. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site |
| 254215 | His6-V5-RNaseR-D280N | His6-V5-RNaseR-D280N encodes an inactive mutant of full-size RNAse R. It has no exonuclease activity. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site |
| 254216 | His6-V5-RNaseR-87-725-D278N | His6-V5-RNaseR-D278N encodes an inactive mutant of full-size RNAse R. It has no exonuclease activity. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site |
| 254217 | His6-V5-RNaseR-87-725-D280A | His6-V5-RNaseR-87-725-D280A encodes an inactive mutant of truncated RNAse R. It has no exonuclease activity. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site |
| 254218 | His6-V5-RNaseR-87-725-D278A | His6-V5-RNaseR-87-725-D278A encodes an inactive mutant of truncated RNAse R. It has no exonuclease activity. It has both an N-terminal 6x HIS tag and a V5 tag ahead of a TEV cleavage site |