Targeting the METTL1/m7G axis as a therapeutic strategy in myeloid leukemia.
Ren L, Zhang H, Bobileva O, Nai F, Bi H, Chan A, Baker GE, Dong L, Guarin D, Hu W, Li W, Leite I, Wang X, Zhang X, Xue M, Wang H, Qin H, Wu X, Ghoda L, Xu L, Zhang B, Li L, Wunderlich M, Mulloy JC, Jones CL, O'Leary SE, Li H, Rosen ST, Chen CD, Heisterkamp N, Perry JJP, Nam Y, Chen J, Caflisch A, Li X, Su R
Blood. 2026 Jun 18;147(25):3069-3085. doi: 10.1182/blood.2025030336.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254416 | lenti-hygro-sgMETTL1-3 | Mammalian expression plasmid encoding an sgRNA targeting human METTL1. |
| 254417 | lenti-hygro-sgMETTL1-6 | Mammalian expression plasmid encoding an sgRNA targeting human METTL1. |
| 254418 | lenti-hygro-M-sgMettl1-3 | Mammalian expression plasmid encoding an sgRNA targeting mouse Mettl1. |
| 254419 | lenti-hygro-M-sgMettl1-4 | Mammalian expression plasmid encoding an sgRNA targeting mouse Mettl1. |
| 254420 | pMIRNA1-METTL1-msg3-WT | Mammalian expression plasmid encoding human METTL1 (sgMETTL1-3-resistant) fused to a 3_FLAG tag. |
| 254421 | pMIRNA1-METTL1-msg6-WT | Mammalian expression plasmid encoding human METTL1 (sgMETTL1-6-resistant) fused to a 3_FLAG tag. |
| 254422 | pMIRNA1-METTL1-msg3-EIR107-109AAA | Mammalian expression plasmid encoding mutant human METTL1 (sgMETTL1-3-resistant) fused to a 3_FLAG tag. |
| 254423 | pMIRNA1-METTL1-msg6-EIR107-109AAA | Mammalian expression plasmid encoding mutant human METTL1 (sgMETTL1-6-resistant) fused to a 3_FLAG tag. |
| 254424 | pMIRNA1-METTL1-WT | Mammalian expression plasmid encoding human METTL1 fused to a 3_FLAG tag. |
| 254425 | pSIN4-HCK | Mammalian expression plasmid encoding human HCK fused to a 3_FLAG tag. |
| 254426 | pLKO.1-shtRNA-Phe(GAA)-1 | Mammalian expression plasmid encoding an shRNA targeting tRNA-Phe(GAA). |
| 254427 | pLKO.1-shtRNA-Phe(GAA)-2 | Mammalian expression plasmid encoding an shRNA targeting tRNA-Phe(GAA). |
| 254428 | pLKO.1-deltaU6-tRNA-Phe(GAA) 1-4 | Mammalian expression plasmid encoding human tRNA-Phe(GAA) isodecoder 1-4. |