NLRP12 interacts with NLRP3 to block the activation of the human NLRP3 inflammasome.
Coombs JR, Zamoshnikova A, Holley CL, Maddugoda MP, Teo DET, Chauvin C, Poulin LF, Vitak N, Ross CM, Mellacheruvu M, Coll RC, Heinz LX, Burgener SS, Emming S, Chamaillard M, Boucher D, Schroder K
Sci Signal. 2024 Jan 23;17(820):eabg8145. doi: 10.1126/scisignal.abg8145. Epub 2024 Jan 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254942 | pEF6_hNLRP10-mCherry (KS300) | Complete human NLRP10 (UniProt Q86W26) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259223 | pEF6_mP3N12L3-mCherry (KS408) | Murine NLRP12/NLRP3 chimeric protein (NLRP3 PYD and LRR, NLRP12 NACHT) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259224 | pEF6_mP3N12[delLRR]-mCherry (KS409) | Murine NLRP12/NLRP3 truncated chimeric protein (NLRP3 PYD, NLRP12 NACHT, delLRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259225 | pEF6_mCherry (KS222) | mCherry in pEF6 for mammalian and bacterial expression. |
| 259226 | pEF6_hNOD1-mCherry (KS299) | Canonical human NOD1 (UniProt Q9Y239-1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259227 | pEF6_hCIITA-mCherry (KS328) | Human CIITA with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains G500A and Q900R (common variants) and P522H relative to UniProt P33076-1. |
| 259228 | pEF6_hNLRP2-mCherry (KS240) | Human NLRP2 with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains a G457V substitution relative to UniProt Q9NX02-1. |
| 259229 | pEF6_hNLRP3-mCherry (KS326) | hNLRP3 with C-term mCherry in pEF6 for mammalian and bacterial expression. Construct initiates at the alternative methionine (Met3), lacking residues 1-2 (MK) relative to canonical hNLRP3 (Q96P20-1). |
| 259230 | pEF6_hNLRP3[delLRR]-mCherry (KS551) | Human NLRP3 (UniProt Q96P20-1 residues 3-651) lacking the LRR domain with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259231 | pEF6_hNLRP3(WalkerAB)-mCherry (KS559) | Human NLRP3 ATPase-deficient (Walker AB) mutant with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259232 | pEF6_hNLRP3(D303N)-mCherry (KS738) | Human NLRP3 D303N gain-of-function mutant with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. D303 corresponds to D305 of the canonical human NLRP3 (UniProt Q96P20-1). |
| 259233 | pEF6_hNLRP4-mCherry (KS241) | Human NLRP4 (NP_604393.2) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains E383D, P390Q, L774M and Q925L relative to the canonical NLRP4 (UniProt Q96MN2-1). |
| 259234 | pEF6_hNLRP5-mCherry (KS292) | Human NLRP5 (GenBank AB527195.1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains a 19aa deletion (E208-Q226) relative to the canonical NLRP5 (UniProt P59047). |
| 259235 | pEF6_hNLRP6-mCherry (KS301) | Canonical human NLRP6 (UniProt P59044-1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259236 | pEF6_hNLRP7-mCherry (KS293) | Canonical human NLRP7 (UniProt Q8WX94-1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259237 | pEF6_hNLRP8-mCherry (KS294) | Canonical human NLRP8 (UniProt Q86W28-1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259238 | pEF6_hNLRP9-mCherry (KS295) | Human NLRP9 with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains C912Y relative to UniProt Q7RTR0-1. |
| 259240 | pEF6_hNLRP11-mCherry (KS359) | Human NLRP11 with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Contains A188S (common variant) relative to the canonical NLRP11 (UniProt P59045-1). |
| 259241 | pEF6_hNLRP12-mCherry (KS242) | Canonical human NLRP12 (UniProt P59046-1) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259242 | pEF6_hNLRP13-mCherry (KS297) | Complete human NLRP13 (UniProt Q86W25) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259244 | pEF6_hCIITA-V5 (KS554) | Human CIITA with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. Contains G500A, Q900R (common variants) and P522H relative to the canonical CIITA (UniProt P33076-1). |
| 259245 | pEF6_hNLRP12-V5 (KS555) | Canonical human NLRP12 (UniProt P59046-1) with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. |
| 259246 | pEF6-hNLRP12(P210L)-V5 (KS888) | Human NLRP12 carrying the patient-associated mutation P210L with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. |
| 259247 | pEF6_hNLRP12(H304Y)-V5 (KS889) | Human NLRP12 carrying the patient-associated mutation H304Y with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. |
| 259248 | pEF6_mNlrp3-mCherry (KS277) | Canonical murine Nlrp3 (UniProt Q8R4B8) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259249 | pEF6_mNlrp3[delLRR]-mCherry (KS280) | Murine Nlrp3 lacking the LRR domain with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259250 | pEF6_mNlrp3(WalkerAB)-mCherry (KS287) | Murine Nlrp3 ATPase-deficient (Walker AB) mutant with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Walker A: G227A, K228A, T229A. Walker B: D298A, D301A, E302A. |
| 259251 | pEF6_mNlrp12-mCherry (KS278) | Canonical murine Nlrp12 (UniProt E9Q5R7) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259252 | pEF6_mNlrp12[delLRR]-mCherry (KS279) | Murine Nlrp12 lacking the LRR domain with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259253 | pEF6_mNlrp12(WalkerAB)-mCherry (KS847) | Murine Nlrp12 ATPase-deficient (Walker AB) mutant with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. Walker A: G222A, K223A, S224A. Walker B: D294A, D297A. |
| 259254 | pEF6_mP12N12L3-mCherry (KS282) | Murine NLRP12/NLRP3 chimeric protein (NLRP12 PYD and NACHT, NLRP3 LRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259255 | pEF6_mP12N3L3-mCherry (KS284) | Murine NLRP12/NLRP3 chimeric protein (NLRP12 PYD, NLRP3 NACHT and LRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259256 | pEF6_mP12N3L12-mCherry (KS406) | Murine NLRP12/NLRP3 chimeric protein (NLRP12 PYD and LRR, NLRP3 NACHT) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259257 | pEF6_mP3N3L12-mCherry (KS283) | Murine NLRP12/NLRP3 chimeric protein (NLRP3 PYD and NACHT, NLRP12 LRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259258 | pEF6_mP3N12L12-mCherry (KS281) | Murine NLRP12/NLRP3 chimeric protein (NLRP3 PYD, NLRP12 NACHT and LRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |
| 259814 | pEF6_mP12N3[delLRR]-mCherry (KS407) | Murine NLRP12/NLRP3 truncated chimeric protein (NLRP12 PYD, NLRP3 NACHT, delLRR) with a C-terminal mCherry tag in pEF6 for mammalian and bacterial expression. |