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Protocol for CRISPR genome editing in S. cerevisiae using PCR-based guide insertion.

Rostamian H, Madden EW, Kaplan FM, Kim RS, Isom DG, Strahl BD
STAR Protoc. 2026 Jul 28;7(3):104751. doi: 10.1016/j.xpro.2026.104751. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
254712pML104-KanMX-sgRNAv2Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Antibodies from Article