Cas12a-knock-in mice for multiplexed genome editing, disease modelling and immune-cell engineering.
Tang K, Zhou L, Tian X, Fang SY, Vandenbulcke E, Du A, Shen J, Cao H, Zhou J, Chen K, Kim HR, Luo Z, Xin S, Lin SH, Park D, Yang L, Zhang Y, Suzuki K, Majety M, Ling X, Lam SZ, Chow RD, Ren P, Tao B, Li K, Codina A, Dai X, Shang X, Bai S, Nottoli T, Levchenko A, Booth CJ, Liu C, Fan R, Dong MB, Zhou X, Chen S
Nat Biomed Eng. 2025 Aug;9(8):1290-1308. doi: 10.1038/s41551-025-01371-2. Epub 2025 Mar 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 256628 | pKT02_MSCV-hU6-BbsI-cPPT-EFS-Cre-mScarlet | Retroviral vector for delivery of AsCas12a guides and expressing Cre and mScarlet using EFS promoter |
| 256629 | pKT07_AAV-hU6-AsDR-EFS-Cre-T2A-hNGFR | AAV vector for delivery of AsCas12a guides and expressing Cre and hNGFR using EFS promoter |
| 256630 | pKT07_AAV-hU6-AsDR-crTSG1-EFS-Cre-T2A-hNGFR | AAV vector for delivery of AsCas12a crTSG1 guide array and expressing Cre using EFS promoter |
| 256631 | pGW004_pAAV-hU6-SapI-hTBG-Fluc-P2A-Cre | AAV vector for delivery of Cas9 guide and expressing Fluc and Cre using hTBG promoter |
| 256632 | pRK16b_MSCV-hU6P-LbDR-BbsI-cPPT-EFS-mScarlet | Retroviral vector for delivery of LbCas12a guides and expressing mScarlet using EFS promoter |
| 256633 | pRC167_Lenti-hU6-AsDR-BsmBI-EF1a-mCherry-2A-Puro | Lentiviral vector for delivery of AsCas12a guides and expressing mCherry using EFS promoter |