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PHIP suppresses NuRD to enable the growth of SWI/SNF-mutant cancers.

Malone HA, Myers JA, Gruss EG, Morgan MA, Friske JD, McCarty TC, Navarro JJ, Robinson S, Halliburton RL, Kietlinska SJ, De Luna Vitorino FN, Hansen BS, Pruett-Miller SM, Garcia BA, Roussel MF, Partridge JF, Roberts CWM
Nat Commun. 2026 Apr 7;17(1):2877. doi: 10.1038/s41467-026-70699-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
259656XLone-Strep-tag-2xHA-PHIPPiggyBac expression for doxycycline inducible expression of Strep-tag and HA-tagged PHIP
259657XLone-Strep-tag-2xHA-delta-H-Box-PHIPPiggyBac expression for doxycycline inducible expression of Strep-tag and HA-tagged PHIP [H-Box deletion mutant]
259659LentiCRISPR-puro-sgPHIP1All-in-one lentiviral transfer vector designed to deliver and express both Cas9 nuclease and sgRNA targeting PHIP (sgPHIP1)
259660LentiCRISPR-puro-sgPHIP2All-in-one lentiviral transfer vector designed to deliver and express both Cas9 nuclease and sgRNA targeting PHIP (sgPHIP2)
259662LentiCRISPR-mCherry-sgPHIP2All-in-one lentiviral vector designed to deliver and express both Cas9 nuclease and sgRNA targeting PHIP (sgPHIP2), with added feature for monitoring (expression of mCherry)
259663LentiCRISPR-mCherry-sgAAVS1All-in-one lentiviral vector designed to deliver and express both Cas9 nuclease and sgRNA targeting AAVS1 (sgAAVS1), with added feature for monitoring (expression of mCherry)
259664LentiCRISPR-mCherry-sgLacZAll-in-one lentiviral vector designed to deliver and express both Cas9 nuclease and sgRNA targeting LacZ (sgLacZ), with added feature for monitoring (expression of mCherry)

Antibodies from Article