Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1.

Zhong Y, Sahan AZ, Shao Z, Zhang QY, Zhou X, Haggett JG, Koshizuka K, Myers SA, Gutkind JS, Zhang J
Nat Chem Biol. 2026 May 14. doi: 10.1038/s41589-026-02188-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
260296	pcDNA3-Cyto-mCherry-TerminaTOR	Genetically encoded mTORC1 inhibitor.
260297	pcDNA3-Lyso-mCherry-TerminaTOR	Genetically encoded mTORC1 inhibitor, targeted to lysosome membrane.
260298	pcDNA3-Nuc-mCherry-TerminaTOR	Genetically encoded mTORC1 inhibitor, targeted to nucleus.
260299	pcDNA3-Mito-mCherry-TerminaTOR	Genetically encoded mTORC1 inhibitor, targeted to outer mitochondrial membrane.
260300	pcDNA3-Mito-TORCAR	Genetically encoded FRET-based biosensor for monitoring mTORC1 kinase activity; targeted to outer mitochondrial membrane.
260301	pcDNA3-Lyso-smuRFP-TerminaTOR	Lysosome-targeted, genetically encoded mTORC1 inhibitor; tagged with smuRFP.
260302	pLenti-TetOn-Cyto-mCherry-TerminaTOR	Lentiviral vector for inducible expression of genetically encoded mTORC1 inhibitor.
260304	pLenti-TetOn-Nuc-mCherry-TerminaTOR	Lentiviral vector for inducible expression of genetically encoded mTORC1 inhibitor targeted to the nucleus.