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pLenti-TetOn-Cyto-mCherry-TerminaTOR
(Plasmid #260302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260302 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 7782
  • Total vector size (bp) 8982
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-TerminaTOR
  • Species
    Synthetic
  • Insert Size (bp)
    1200
  • Mutation
    PRAS40 114-245; S183A
  • Promoter TREG3
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-TetOn-Cyto-mCherry-TerminaTOR was a gift from Jin Zhang (Addgene plasmid # 260302 ; http://n2t.net/addgene:260302 ; RRID:Addgene_260302)

  • For your References section:

    Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1. Zhong Y, Sahan AZ, Shao Z, Zhang QY, Zhou X, Haggett JG, Koshizuka K, Myers SA, Gutkind JS, Zhang J. Nat Chem Biol. 2026 May 14. doi: 10.1038/s41589-026-02188-z. 10.1038/s41589-026-02188-z PubMed 42135521
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