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V-SWITCH: A single-vector OFF-to-ON fluorescent reporter of live RNA virus infections

Cid-Rosas M, Grunberg M, Matía A, Lee S-C, Woosley H, Pradeep S, Kanwar S, Khadka S, Mehta S, Hüttenhain R, Turon-Lagot V, Arias C
bioRxiv 2026.04.08.717260 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
262011DENV-SWITCHLentiviral OFF-to-ON split-mNeonGreen reporter for DENV NS2B/3 protease activity during live infection; nuclear TagBFP marks reporter-expressing cells.
262012ZIKV-SWITCHLentiviral OFF-to-ON split-mNeonGreen reporter for ZIKV NS2B/3 protease activity during live infection; nuclear TagBFP marks reporter-expressing cells.
262013WNV-SWITCHLentiviral OFF-to-ON split-mNeonGreen reporter for WNV NS2B/3 protease activity during live infection; nuclear TagBFP marks reporter-expressing cells.
262014OC43-SWITCHLentiviral OFF-to-ON split-mNeonGreen reporter for HCoV-OC43 nsp5/3CLpro activity using the nsp14/nsp15 junction; nuclear TagBFP marks reporter expression.

Antibodies from Article