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DENV-SWITCH
(Plasmid #262011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262011 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pWPI
  • Backbone manufacturer
    Didier Trono laboratory
  • Total vector size (bp) 13205
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mNG(1-10)-PCS-Sec61bTM-BlasticidineR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1360
  • Promoter EF-1a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer EF-1a_forward_primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mNG(11)-BFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    909
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer CMV_forward_primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.04.08.717260 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DENV-SWITCH was a gift from Carolina Arias (Addgene plasmid # 262011 ; http://n2t.net/addgene:262011 ; RRID:Addgene_262011)

  • For your References section:

    V-SWITCH: A single-vector OFF-to-ON fluorescent reporter of live RNA virus infections. Cid-Rosas M, Grunberg M, Matía A, Lee S-C, Woosley H, Pradeep S, Kanwar S, Khadka S, Mehta S, Hüttenhain R, Turon-Lagot V, Arias C. bioRxiv 2026.04.08.717260 10.64898/2026.04.08.717260
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